Composite

Part:BBa_K4662045

Designed by: Julia Wyss   Group: iGEM23_UZurich   (2023-10-09)


RHlI assembly

Constitutive expression of RhlI which directs the synthesis of N-(butanoyl)-homoserine lactone (C4-HSL).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 255
    Illegal EcoRI site found at 732
    Illegal PstI site found at 693
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 255
    Illegal EcoRI site found at 732
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 693
    Illegal NotI site found at 874
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 255
    Illegal EcoRI site found at 732
    Illegal BamHI site found at 726
    Illegal XhoI site found at 883
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 255
    Illegal EcoRI site found at 732
    Illegal PstI site found at 693
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 255
    Illegal EcoRI site found at 732
    Illegal PstI site found at 693
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

For characterisation information about the part, check BBa_K4662044.

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Categories
Parameters
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