Composite

Part:BBa_K4662038

Designed by: Julia Wyss   Group: iGEM23_UZurich   (2023-10-09)


LasB with CFP

Level 1 assembly with: BBa_R0079: Promoter which is activated when RhlR (transcription factor) BBa_C0071: bound with RhlI (auto-inducer molecule) binds BBa_K4662044:

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 405
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 405
    Illegal NheI site found at 58
    Illegal NheI site found at 299
    Illegal NotI site found at 547
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 405
    Illegal BamHI site found at 399
    Illegal XhoI site found at 556
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 405
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 405
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Since this part was never used alone further information can be found under: BBa_K4662042

Usage and Biology

With our complete characterization of the rhl-las quorum sensing hybrid system, the CFP moiety can be switched to any protein of interests for rhl dependent expression


Remark

Specific protocols for assembly and testing can be found on our wiki https://2023.igem.wiki/uzurich/experiments

If any questions arise or additional information is needed, we can provide a more extensive list of experiments that we can share if required.

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