Composite

Part:BBa_K4662028

Designed by: Julia Wyss   Group: iGEM23_UZurich   (2023-10-06)


RhlR assembly

RhlR assembly with constitutive anderson promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

RhlR is an enzyme that acts as a transcription factor, as soon as the concentration of C4-HSL reaches a certain threshold and can bind to RhlR. In the bound state, RhlR gets active and induces transcription of specific genes.


Characterization

Part was only characterized in a level 2 construct.

For more information on it usage BBa_K4662047 and BBa_K4662042

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Categories
Parameters
None