Part:BBa_K4329009
Quorum sensing suicidal system
what is it
The quorum-sensing suicidal system consists of three parts: LUXR, CRISPR/Cas9, and sgRNA. The LUXR protein is regulated by the T7 promoter, lac operator, and T7 terminator. CRISPR/Cas9 is regulated by a quorum-sensing promoter and quorum-sensing terminator. sgRNA is regulated by the constitutive promoter and terminator.
Usage and Biology
At first, if the IPTG is absent, the RNA ligase in the system will converge with the T7 promoter which can not lead to the production of mRNA. Thus, protein and enzyme production will be inhibited. However, in difficult situations, IPTG could be present in the natural environment, so we have considered a double safety system. If LUXR detects the presence of AHL, a quorum-sensing chemical sent out by E.Coli, a quorum-sensing promoter will be activated. Later, the promoter will activate the CRISPR/Cas9 system targeting the ATP ligase of E.coli. Consequently, without ATP, the bacteria will be forced to die.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 5438
Illegal NheI site found at 5461 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 5485
- 1000COMPATIBLE WITH RFC[1000]
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