Coding

Part:BBa_K4252008

Designed by: Yuhan Wu   Group: iGEM22_HNU_China   (2022-09-26)


Phosphate ABC transporter membrane subunit PstB.

The phosphate (Pi)-specific transport system of Escherichia coli (Pst) is a typical ABC transport system composed of four different proteins. PstB, the fourth gene in the Pst operon is able to express the protein that binds ATP and energizes the transport.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 577


Introduction

PstB determine pstB protein that is the catalytic subunit and interact on the cytoplasmic side, which couples the energy of ATP hydrolysis to control the open and close of phosphate channel by the alpha-helix domains of PstA and PstC. And phosphate molecule can across the channel by the salt bridge composed of Arg and Glu.
For further imformation, go to Pst part (BBa_K4252009).

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