Composite

Part:BBa_K4245210

Designed by: Michelle Jing, Daeun Lee, Richard Jiang, Sishnukeshav Balamurali, Christina Yi, Janet Standeven   Group: iGEM22_Lambert_GA   (2022-10-09)


T7 regulated miRNA1-broccoli-spacer
This part is the T7 regulated miRNA1-broccoli-spacer, designed to test the efficacy of the hsa-miR-1-3p RCT Padlock Probe (BBa_K4245201). We were unsure if the inclusion of a spacer sequence (BBa_K4245160) affected the folding of the broccoli aptamer (BBa_K3380153). The part was designed to imitate the transcript produced when Rolling Circle Transcription (RCT) is applied to hsa-miR-1-3p RCT Padlock Probe.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 137
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None