Part:BBa_K4245008

hsa-miR-126-3p

This part is the sequence for hsa-miR-126-3p, an miRNA isolated from Homo sapiens. This miRNA acts as a downregulated biomarker for coronary artery disease and is therefore potentially useful for the early detection of this condition. miRNA-126-3p is known to regulate vascular inflammation and is associated with the activation of vascular endothelial growth factor signaling in the endothelium. From multiple studies, miRNA is seen to be consistently and significantly downregulated as the severity of coronary artery disease increases (Wang et al., 2017). Thus, utilizing miRNA-126-3p can have the potential to follow up with patients on the development of CAD, acting as a biomarker that Lambert iGEM can utilize.

The Lambert_GA 2022 team developed a set of padlock probes to use the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for BBa_K4245102 and BBa_K4245109, the 3' arm for hsa-miR-126-3p and 5' arm for hsa-miR-126-3p, and as the target sequence for BBa_K4245203, the hsa-miR-126-3p RCA Padlock Probe.

When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.

References:

Wang, X., Lian, Y., Wen, X., Guo, J., Wang, Z., Jiang, S., & Hu, Y. (2017). Expression of miR-126 and its potential function in coronary artery disease. African health sciences, 17(2), 474–480. https://doi.org/10.4314/ahs.v17i2.22

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]