Composite

Part:BBa_K4244019

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-09)


pTlpA ccdA-B

pTIpA, regulated by TlpA, is upstream the ccdA antitoxin. When temperature goes below 37 °C, this leads to a decreased expression of the antitoxin and the toxin ccdB leads to the microorganism’s death.

This construct consists of the toxin ccdB with the placUV5 promoter (BBa_K4244018) which is reversed, together with pTlpA and the antitoxin ccdA (BBa_K4244016) and the TlpA (BBa_K4244017). This construct has been tested in E.coli Nissle 1917 with a temperature sensitive survival assay. Unfortunately, the experiment did not show the expected results (decrease of colony number with decrease of temperature).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 678
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1028
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2971
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 678
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 678
    Illegal NgoMIV site found at 568
    Illegal AgeI site found at 3274
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None