Plasmid
Part:BBa_K4188003
Designed by: Tobias Bröker Group: iGEM22_WWU_Muenster (2022-09-28)
pSB3CY_Leu shuttle vector with leucine synthase
This is the ready-to-use version of the pSB3CY_shuttlevektor backbone (BBa_K4188001) for Saccharomyces cerevisiae where the aeBlue chromoprotein is exchanged with the leucine synthase LEU2.It can be used for MoClo like the pSB3C01 backbone.
Usage and Biology
Test transformation
After a successful golden gate exchange of the aeBlue chromoprotein through the leucine synthase, the resulting vector pSB3CY_Leu was transformed into the yeast Saccharomyces cerevisiae.
Figure 1:
left picture: Saccharomyces cerevisiae colonies formed from 100µl plated transformation mixture
right picture: Saccharomyces cerevisiae colonies formed from 10µl plated from the same transformation mixture
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7094
Illegal XbaI site found at 3425
Illegal SpeI site found at 3790
Illegal PstI site found at 1105 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7094
Illegal NheI site found at 5780
Illegal SpeI site found at 3790
Illegal PstI site found at 1105
Illegal NotI site found at 5721 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7094
Illegal BamHI site found at 2441 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7094
Illegal XbaI site found at 3425
Illegal SpeI site found at 3790
Illegal PstI site found at 1105 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7094
Illegal XbaI site found at 3425
Illegal SpeI site found at 3790
Illegal PstI site found at 1105
Illegal AgeI site found at 793
Illegal AgeI site found at 905
Illegal AgeI site found at 2083
Illegal AgeI site found at 2406
Illegal AgeI site found at 4697 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1378
Illegal BsaI site found at 7100
Illegal BsaI.rc site found at 1099
Illegal SapI site found at 1082
Illegal SapI.rc site found at 6
[edit]
Categories
Parameters
//chassis/eukaryote/yeast
//collections/shuttle_vectors/level2
//collections/shuttle_vectors/level2
None |