Coding

Part:BBa_K4175013

Designed by: Li Xianxiu   Group: iGEM22_ZJUintl-China   (2022-09-29)


truncated human extracellular IL-6R

This part is the truncated version of extracellular human IL-6R (aa 1-309). We joined this part with Notch core domain (BBa_K4175001) and ZF_GAl4_KRAB (BBa_K2446037) (Fig 1). We used this truncated version instead of complete extracellular part of IL-6R (BBa_K4175002) because the Notch core domain already contains transmembrane domain. Therefore, we excluded the transmembrane and the hinge part of IL-6R. For more details for IL-6R, please visit BBa_K4175002. For more details for the composite part, IL-6R-Notch-Gal4KRAB, please visit BBa_K4175010.

Figure 1. IL-6R-Notch-Gal4KRAB in CAR-T cells under low and high IL-6 concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 640
    Illegal PstI site found at 817
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 640
    Illegal PstI site found at 817
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 717
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 640
    Illegal PstI site found at 817
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 640
    Illegal PstI site found at 817
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None