Part:BBa_K4133004

RBS_tuf33 (Lactobacillus plantarum)

This ribosomal binding site (RBS) was originally taken from the Ptuf33 promoter sequence reported in the work of Tauer and collaborators (2014). It has been previously used for recombinant gene expression in Lactobacillus plantarum.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 19
  

Usage and Biology

This RBS was derived from the pSIP409, a high-level inducible vector, and was used in the expression constructs designed by Sørvig and collaborators (2005). Furthermore, as changes in the length and/or sequence between a Shine-Dalgarno-sequence (SDS) and the start codon can affect expression, it has been found that the optimal spacer between the SDS and the start codon in L. plantarum consists of 8 nucleotides.

References

Sørvig, E., Mathiesen, G., Naterstad, K., Eijsink, V. G. H., & Axelsson, L. (2005). High-level, inducible gene expression in Lactobacillus sakei and Lactobacillus plantarum using versatile expression vectors. Microbiology, 151(7), 2439–2449. https://doi.org/10.1099/mic.0.28084-0

Tauer, C., Heinl, S., Egger, E., Heiss, S., & Grabherr, R. (2014). Tuning constitutive recombinant gene expression in Lactobacillus plantarum. Microbial Cell Factories, 13(1). https://doi.org/10.1186/s12934-014-0150-z