Composite

Part:BBa_K4115037

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)


J23107-B0034-SacC-B0015


Designed for constitutively expression of SacC protein in E. coli.

Usage and Biology

Constitutive expression of the SacC protein in E. coli is expected to confer the ability to efficiently utilize extracellular sucrose produced by cyanobacteria.Also involved in screening for constitutive promoters suitable for the project.
See BBa_K4115017 for further details.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 498
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1370
    Illegal PstI site found at 498
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1413
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 498
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 498
    Illegal NgoMIV site found at 757
    Illegal AgeI site found at 479
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None