![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
Part:BBa_K4115014
Designed by: Shenxi Cao Group: iGEM22_ShanghaiTech_China (2022-09-21)
PnifH(with RBS)
The promoter of the nifH coding sequence on the nitrogen-fixing gene cluster is regulated by the nifA protein.
Usage and Biology
The nitrogen-fixing gene cluster comes into play due to the downstream protein expression of PnifH in the original A. caul genome[1]. The PnifH fragment was added to our designed system and sfGFP (BBa_K4115000) was expressed downstream to verify whether the artificially designed system could regulate nif gene cluster as expected in A. caul.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 272
[1]Ryu, MH., Zhang, J., Toth, T. et al. Control of nitrogen fixation in bacteria that associate with cereals. Nat Microbiol 5, 314–330 (2020). https://doi.org/10.1038/s41564-019-0631-2
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Categories
Parameters
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