Composite
Pre A3

Part:BBa_K4060102

Designed by: CHEN YAN-ZHEN   Group: iGEM21_NYCU-Taipei   (2021-09-30)


[Pre A3] a RFP gene ligated before double terminator

a RFP gene ligated before double terminator

The gene fragment Pre A3 is composed of a Red Fluorescent Protein(BBa_E1010) and a double terminator(BBa_B0015). We digested the vector fragment BBa_B0015 with XbaI and EcoRI and the insert fragment BBa_E1010 with EcoRI and SpeI. We ligated these fragments together to form Pre A3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None