Part:BBa_K4047036

pGEM-tal

Plasmid for overexpressing transaldolase in Synechococcus elongatus PCC 7942; contains all the components of pAM2991 in addition to our gene

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 7968
  Illegal XbaI site found at 2
  Illegal XbaI site found at 2546
  Illegal XbaI site found at 2860
  Illegal PstI site found at 14
  Illegal PstI site found at 2558
  Illegal PstI site found at 5013
  Illegal PstI site found at 5815
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 7968
  Illegal NheI site found at 2673
  Illegal NheI site found at 8658
  Illegal PstI site found at 14
  Illegal PstI site found at 2558
  Illegal PstI site found at 5013
  Illegal PstI site found at 5815
  Illegal NotI site found at 515
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 7968
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 7968
  Illegal XbaI site found at 2
  Illegal XbaI site found at 2546
  Illegal XbaI site found at 2860
  Illegal PstI site found at 14
  Illegal PstI site found at 2558
  Illegal PstI site found at 5013
  Illegal PstI site found at 5815
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 7968
  Illegal XbaI site found at 2
  Illegal XbaI site found at 2546
  Illegal XbaI site found at 2860
  Illegal PstI site found at 14
  Illegal PstI site found at 2558
  Illegal PstI site found at 5013
  Illegal PstI site found at 5815
  Illegal NgoMIV site found at 1043
  Illegal NgoMIV site found at 3454
  Illegal NgoMIV site found at 8493
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 198
  Illegal BsaI site found at 2068
  Illegal BsaI site found at 9117
  Illegal BsaI.rc site found at 4839
  Illegal BsaI.rc site found at 5605
  Illegal SapI site found at 3756