Part:BBa_K4032005

α-galactosidase-RFP

This part contains the gene of the α-galactosidase-RFP fusion protein.

Usage and Biology

This enzyme catalyzes the hydrolysis of terminal, non-reducing α-D-galactose residues in α-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. For more information, see UniProt EC:3.2.1.22.

Red fluorescence of RFP is observed not only with a fluorescence microscope but also under the natural light source.

The lac promoter, the RBS and the double terminator are added to this part, forming BBa_K4032101.

Design

This part expresses the α-galactosidase-RFP fusion protein.

α-galactosidase is from E. coli and RFP is from BBa_J04450.

We removed the stop codon of the α-galactosidase gene and the start codon of the RFP gene and connected the two ends.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1905
  Illegal AgeI site found at 2017
  
• 1000
  COMPATIBLE WITH RFC[1000]