Coding

Part:BBa_K3853000

Designed by: Peng Luo   Group: iGEM21_CPU_CHINA   (2021-09-17)


Manganese Peroxidase (MnP)


Manganese Peroxidase (MnP) is a highly glycosylated lignin peroxidase with heme. It can oxidize Mn2+ to Mn3+, which would be chelated by ligands like oxalic acid. And the Mn3+-ligand chelate compound would be released outside the enzyme for further degrading lignin or other refractory chemicals.This gene comes from Phanerochaete chrysosporium. Using the Thermo GeneArt online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in Pichia pastoris. The introduction of this gene helps our system degrade PE effectively.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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