Coding

Part:BBa_K3722004

Designed by: Ruozhou Wang   Group: iGEM21_NWU-CHINA-A   (2021-09-28)


A linker sequence in fusion protein Fre-Linker-SttH

A linker sequence belongs to our fusion protein Fre-Linker-SttH, coding a rigid linker between Fre and SttH.


Biology

It is a rigid peptide, which is used to completely isolate the two connected proteins and maintain their independent functions, because some flexible peptides will cause the expression of the fusion protein to decrease or the activity to be lost. It should be noted that rigid peptides cannot be used for two interacting proteins.


Usage

The part was used to construct SttH and Fre fusion enzyme to enhance the solubility and activity of SttH. Due to the limited time, we did not characterize this part. As a result, there is a lack of data about this part.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



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Categories
Parameters
None