Coding

Part:BBa_K3634008

Designed by: Laurence Seeley   Group: iGEM20_St_Andrews   (2020-08-05)


Phycocyanobilin:Ferredoxin Oxidoreductase (pcyA) (Optimised for E.coli)

The gene pcyA codes for the functional phycocyanobilin:ferredoxin oxidoreductase protein, the second enzyme required for the conversion of heme to the blue phycobilin phycocyanobilin (PCB); a chromophore required for activation of some two component light-sensing systems (TCS) such as the ccaS/ccaR system in Synechocystis sp. PCC 6803. The protein catalyses the four-electron reduction of biliverdin IXα, previously produced from heme by heme oxygenase, to PCB, which then binds to the appropriate membrane associated histidine kinase of the TCS allowing activation and expression of the output gene in the presence of an activating wavelength of light. The part should be used alongside BBa_K3634006 and BBa_K3634007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None