Tag
Part:BBa_K3606042
Designed by: Zhenru Zhang Group: iGEM20_Fudan (2020-10-23)
Nsp4 signal peptide
NSP4 signal peptide for protein secretion in bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
- 1000COMPATIBLE WITH RFC[1000]
NSP4 is a signal peptide in E.coli. which functions through the type II secretion system, which generally contains two steps. The first step involves some signal peptides targeted to the Sec secretory pathways, which are responsible for transporting the target protein across the inner membrane into the periplasm. For Gram-negative bacteria like E.coli, the second step can then take place once the target protein is in the periplasm, as the intrinsic protein complex GspD creates a pore in the outer cell membrane through which the target protein can be secreted out of the bacteria.
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Categories
Parameters
| None |