Part:BBa_K3505041

LacZa for Bacterial GoldenBraid plasmids

This LacZa moudule is the cargo of the Bacterial GoldenBraid Plasmid Collection.

This LacZa module is flanked by the proper BsaI and BsmBI sites giving ID to the backbones

• pUPD2 backbone BBa_K3505043+ LacZa pUPD2 = BBa_K3505007
• alpha backbone BBa_K3505040+ LacZa α1R = BBa_K3505008
• alpha backbone BBa_K3505040+ LacZa α2 = BBa_K3505009
• omega backbone BBa_K3505042+ LacZa Ω1 = BBa_K3505010
• omega backbone BBa_K3505042+ LacZa Ω2 = BBa_K3505011

Fig.1: The LacZa module flanking sites for every GB pLamid type

Fig.1: The GoldenBraid Garmmar

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 366
  Illegal XbaI site found at 393
  Illegal PstI site found at 405
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 366
  Illegal PstI site found at 405
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 366
  Illegal BamHI site found at 387
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 366
  Illegal XbaI site found at 393
  Illegal PstI site found at 405
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 366
  Illegal XbaI site found at 393
  Illegal PstI site found at 405
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 7
  Illegal BsaI.rc site found at 616
  

References

[1] Alejandro Sarrion-Perdigones, Marta Vazquez-Vilar, Jorge Palací, Bas Castelijns, Javier Forment, Peio Ziarsolo, José Blanca, Antonio Granell, Diego Orzaez (2013). “GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology.” Plant Physiology , 162 (3) 1618-1631; DOI: 10.1104/pp.113.217661