Composite

Part:BBa_K3505027

Designed by: Venetios Michelioudakis, Magdalini Koroxenidou, Foteini Papadaki   Group: iGEM20_Thessaly   (2020-10-20)


PfliC:RBS-lac repressor:terminator


LacI BBa_K3505003uder control of a SCFAs inducible promoter pFLliC BBa_K2924016.

Fig.1:pFliC-LacI-Terminator

Usage and Biology

This Trancriscription Unit (TU) is activated form SCFAs and more specifically from Butyrate. Exressing the LacI protein for the inhibition of an other TU that is lac regulated.


Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in BBa_K3505008 and has overhangs compatible for GoldenBraid cloning.

Verification of cloning

Fig.2:(U=Uncut C=Cut) Restriction Enzyme with BamHI, HpaI Expected bands in bp 2847 + 1348, 4195

Experimental Use and Experinece

This part is used in BBa_K3505036

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None