Coding

Part:BBa_K3425045

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-15)

TEV cleavage site ENLYFQ^G

BBa_K3425045 is the cleavage site of TEV protease (BBa_K3425037, Tobacco Etch Virus protease). This part is codon optimized for E. coli and B. subtilis and thus its nucleotide sequence is distinct from the BBa_K3102041, while the protein sequence is the same: Glu-Asn-Leu-Tyr-Phe-Gln-Gly. Peptide is cleaved between the glutamine and glycine residues.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
//direction/forward
Parameters
chassisE. coli
directionforward
functioncleavage site of TEV protease
proteinpeptide cleavage sequecne