Part:BBa_K3356007

Ketone Production Operon DBHB_b Under the Control of the PLAC Inducible Promoter

This parts entry represents the DBHB_b ketone production operon under the control of the BgaRL lactose inducible system from C. perfringens where the divergent PbgaR BBa_K2992020 and PbgaL BBa_K2992023 promoter sequences, coupled with their native 5’-UTR and RBS sequences BBa_K2992022 and BBa_K2992024 respectively control the transcription of bgaR and the pathway.
The first gene in the KPO is the thiolase gene thl BBa_K2992008, followed by phbB from Cupriavidus necator [1] and finally tesB [2] from Escherichia coli.
Transcriptional termination for this synthetic ketone production operon occurs via Tfdx from C. pasteurianum K2284012.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 411
  Illegal XbaI site found at 1420
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 411
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 411
  Illegal BglII site found at 471
  Illegal BamHI site found at 3527
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 411
  Illegal XbaI site found at 1420
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 411
  Illegal XbaI site found at 1420
  Illegal AgeI site found at 2269
  Illegal AgeI site found at 2941
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3130
  Illegal SapI.rc site found at 3142