Part:BBa_K3222000

T7 promoter with GGG of 3' end

BBa_K3222000 is an improved part of BBa_I746916 which encodes super folder GFP gene (Pedelacq et al. (2006): "Engineering and characterization of a super folder green fluorescent protein", Nature Biotech 24 (1) January 2006). We changed 15A to G to remove Sap1 restriction site with the silent mutation. This part meets both Biobrick standard and Type llS standard.

Contribution: New documentation from Evry_Paris-Saclay 2020

This part is the strong promoter from T7 bacteriophage (taatacgactcactata) with GGG at 3' end.

The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GGG is one of the best +1, +2 and +3 base combinations at the transcription initiation for enhanced promoter strength [1].

References

[1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]