Reporter

Part:BBa_K3190204

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-14)


ZsGreen: Green fluorescent protein

ZsGreen is a basic (constitutively fluorescent) green fluorescent protein published in 1999, derived from Zoanthus sp. and has a molecular weight of 26.1 kDa. ZsGreen is encoding a synthetic green fluorescent protein, that has been modified for high solubility, bright emission, and rapid chromophore maturation. We use ZsGreen as a reporter gene wherein it is coupled to the inducible promoter pFIG1 that is activated when the transcription factor STE12 binds to the promoter to initiate the transcription of the reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None