Part:BBa_K3190203
STE12: Transcription factor downstream MAPK
Yeast respond to mating pheromone by transducing the pheromone signal through a well-studied mitogen-activated protein kinase (MAPK) cascade. The culmination of this kinase cascade differentiates the cell for mating by activating transcription, altering cellular morphology, and arresting the cell in G1 phase. Phosphorylation by either of the two partially redundant kinases Fus3p and Kss1p at the end of the kinase cascade activates the Ste12 transcription factor, resulting in expression of genes involved in mating. Ste12p binds to pheromone response elements (PREs) in the upstream activating sequences of its target genes. The STE12 transcription factor is activated downstream of the MAPK cascade when phosphorylated by Fus3p. It then binds to the pheromone response element (PREs) upstream of the target genes, we therefore exploit this mechanism to make the activated STE12 bind to the promoter of our reporter gene i.e. pFIG1
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 640
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1413
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 640
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 640
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 850
Illegal BsaI.rc site found at 1563
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