Translational_Unit

Part:BBa_K316030

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-20)

LytC cell wall anchoring protein for B. subtilis

The cell wall binding domain of lytC, a N-acetylmuramoyl-L-alanine amidase of B. subtilis, was isolated to be used as a cell wall anchor. As demonstrated by Kobayashi et al. 20001 this domain can be used to target catalytic domians of other proteins as well as peptides to the cell wall of B. subtilis whilst leaving their functionality intact.


This biobrick contains 2-953 of 1491bp of the complete genomic LytC. Full sequence can be found on EMBL[http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+%5BEMBLCDS:CAB15579%5D+-newId] (Acession number AL009126.3)


CWBprotein.PNG

Figure I. Graphical representation of cell wall binding protein.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




References

<biblio>

  1. 1 pmid=14597697

</biblio>

For more information about our project please visit our wiki [http://2010.igem.org/Team:Imperial_College_London].

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