Coding

Part:BBa_K3132003

Designed by: Qiliang Yang   Group: iGEM19_SMMU-China   (2019-10-05)


PIP-VP64

This part is transformed from the part: ZF_PIP_KRAB(TF-KRAB-2) (BBa_K2446045) of iGEM17_Fudan. It is a synthetic transcription factor (SynTF). It contains three core domains from N-terminal to C-terminal: PIP DNA binding domain (DBD), nuclear location sequence and transcription regulating domain. To turn this part from an inhibitory one to an activating one, we use VP64 to displace the KRAB gene. So, we use PIP-VP64 as a specific transcription factor to activate the expression of our downstream synthetic promote.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 475


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Categories
Parameters
None