Composite

Part:BBa_K3081021

Designed by: Xiaoying Qiao   Group: iGEM19_Peking   (2019-10-19)


pBAD-dCas9-J23119-T1

This composite part is the principal design of the inducible CRISPRi system, with assistance of constantly expressed sgRNA (T1) that binds to the complementary coding sequence of mRFP. So it needs to cotransform with a plasmid coding mRFP.Fluorescence is mildly decreased as the concentration of arabinose increases, while the sgRNA NT1BBa_K3081022 has a more severe inhibition than T1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None