Part:BBa_K3020004

umuDC+gfp

Since the expression of umuDC in E. coli is relatively stable and is not affected by the chemicals used in the experiment, the expression level of umuDC can be used to express the cell activity. And the umuDC is an important promoter in the SOS reaction. Therefore, after the introduction of the "umuDC+gfp" line, the fluorescence intensity expressed by E. coli can refer to the biological activity of the cells at this time, and it can be judged whether the normal physiological activity is affected and the degree of influence. Based on this, a related experiment was designed to determine the specific damage degree of E. coli at different concentrations of damaging agents. This line provides clear feedback to E. coli that is damaged and has reduced physiological activity. This line effectively improves the measurement accuracy of E. coli activity

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1091