Part:BBa_K2865010

CMV-AR185-Poly(A)

This is a mammalian eGFP generator which was used to verify the CMV promoter can actually promote the protein AR185 expression and was used as a control of the parts BNP-AR185-Poly(A) (BBa_K2865009). This part contains the CMV promoter sequence followed by AR185-T2A-EGFP (BBa_K2865001), and SV40 polyA terminator (BBa_K2865004). We have clearly description about AR185 and Poly(A) gene.

Experiment

We transfected H9C2 cell with this part by liposome transfection. After 48 hours’ expression, we detected the cells using fluorescence microscope. And we have taken clearly photos. Because of the low cell division rate and cell specificity, we have to admit that the transfection efficiency is low. But in the cell experiment, we have got credible evidence to prove the hypothesis.

 

Figure 1. The fluorescence expression of CMV-AR185-Poly(A) 48 hours later

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 817
  Illegal NgoMIV site found at 964
  Illegal NgoMIV site found at 1899
  
• 1000
  COMPATIBLE WITH RFC[1000]