Composite

Part:BBa_K2807003

Designed by: Ravichandran Divyapoorani   Group: iGEM18_NUS_Singapore-Sci   (2018-09-30)

rAPOBEC1-XTEN-dPspCas13b

rAPOBEC1-XTEN-dPspCas13b is a fused gene that was synthesised by our team using basic parts like rAPOBEC1 and dPspCas13b as well as the linker part, XTEN. This fusion protein targets specific mRNA and makes edits from the base cytosine, C to uracil, U.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3636
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2539
    Illegal BamHI site found at 1591
    Illegal BamHI site found at 4023
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal AgeI site found at 2272
    Illegal AgeI site found at 3148
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 103
    Illegal SapI site found at 625


[edit]
Categories
Parameters
None