Device

Part:BBa_K2719007

Designed by: Armando Cortés Reséndiz & María José Ugarte Orozco   Group: iGEM18_TecCEM   (2018-08-08)


Collagen V - Like Expression Device.

Collagen is optimized for Escherichia coli Rosetta 2-DE3 because it was the only strain modified for the detection of non-canonical amino acids. In this case, collagen has a non-canonical amino acid, specifically Hydroxyproline, in order to obtain a correctly binding between collagen and heparin. This protein was also cloned in pSB1C3 and in it, additionally, was cloned a Red Fluorescent Protein (RFP) to prove that the expression of collagen is taking place in the bacteria. This part contain a T7-lac promoter (BBa_R0184), a RBS (BBa_J611000) and a double TAATAA terminator (BBa_B0015). The prefix and suffix of every part were based on BioBrick RFC10.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1046


[edit]
Categories
Parameters
None