Part:BBa_K2650003
BslA mCherry Expression Cassette
BslA mCherry is a composite part that consists of a transcription terminator (parts B0011 and B1002), a P43 constitutive Bacillus promoter, RBS (Bacillus; AGGAGG; 7 bp upstream of start ATG codon), coding regions for the secretion tag LipA and hydrophobin BslA, a GS linker protein (a very linear protein used to link BslA and mCherry, while still allowing for correct folding), and a coding region for mCherry. There are two terminators to ensure that transcription ends. LipA is a secretion tag that helps mark the protein for secretion from Bacillus cells. BslA is a hydrophobin that binds to a variety of hydrogen bonds and is hydrophobic. The mCherry is an RFP or a red fluorescent protein. The BslA linked to mCherry creates a fusion protein that attaches itself to any hydrogen bond and will have a red fluorescent colour that will be detectable to the naked eye and under UV light.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 187
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1620
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 617
Illegal AgeI site found at 1352 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 785
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