Part:BBa_K256004

NorR

DNA-binding transcriptional activator, B2709, NorR, YgaA, transcriptional regulator of the anaerobic response to reactive nitrogen species

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 655
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 430
  

MIT_MAHE 2020

Summary

Nitric oxide (NO) induces NO-detoxifying enzymes in Escherichia coli suggesting sensitive mechanisms for coordinate control of NO defense genes in response to NO stress. Exposure of E. coli to sub-micromolar NO levels under anaerobic conditions rapidly induces transcription of the NO reductase (NOR) structural genes. This protein is involved in the pathway nitric oxide reduction, which is part of Nitrogen metabolism. It also acts as a transcriptional activator for at least the norVW operon. It binds to the promoter region of norVW, to a consensus target sequence, GT-(N7)-AC, which is highly conserved among proteobacteria.

References

1. Gardner, A. M., Gessner, C. R., & Gardner, P. R. (2003). Regulation of the nitric oxide reduction operon (norRVW) in Escherichia coli. Role of NorR and sigma54 in the nitric oxide stress response. The Journal of biological chemistry, 278(12), 10081–10086. https://doi.org/10.1074/jbc.M212462200

2. Roselle, D. C., & Smith, D. J. (1998). Characterization and nitric oxide release studies of lipophilic 1-substituted diazen-1-ium-1,2-diolates. Journal of controlled release : official journal of the Controlled Release Society, 51(2-3), 131–142. https://doi.org/10.1016/s0168-3659(97)00148-x