Coding

Part:BBa_K2546004

Designed by: Tsu-Wang Sun   Group: iGEM18_NCHU_Taichung   (2018-10-03)


pME60C shuttle vector

This part is an [http://2018.igem.org/Team:NCHU_Taichung/Improve improvement] of pSB1C3 contains BBa_J364007. We transform BBa_K2546004 into Burkholderia cenocepacia Strain 869T2. By interlab protocol: Cell growth, sampling, and assay, we can analyze the BBa_K2546004 work. According our result, BBa_K2546004 work well in Burkholderia cenocepacia Strain 869T2.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5379
    Illegal SpeI site found at 6471
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5379
    Illegal NheI site found at 2246
    Illegal SpeI site found at 6471
    Illegal NotI site found at 1547
    Illegal NotI site found at 2837
    Illegal NotI site found at 5385
    Illegal NotI site found at 6478
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5379
    Illegal BamHI site found at 4230
    Illegal XhoI site found at 4363
    Illegal XhoI site found at 5255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5379
    Illegal SpeI site found at 6471
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5379
    Illegal XbaI site found at 5394
    Illegal SpeI site found at 6471
    Illegal NgoMIV site found at 1464
    Illegal NgoMIV site found at 2445
    Illegal NgoMIV site found at 2590
    Illegal NgoMIV site found at 2594
    Illegal NgoMIV site found at 2801
    Illegal NgoMIV site found at 3023
    Illegal NgoMIV site found at 3269
    Illegal AgeI site found at 216
    Illegal AgeI site found at 540
    Illegal AgeI site found at 1752
    Illegal AgeI site found at 6181
    Illegal AgeI site found at 6293
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1245
    Illegal BsaI.rc site found at 1849
    Illegal SapI site found at 1620


Pictures

  • Compare the RFP in BBa_K2546004(bottom 2 tube) and BBa_J044500-pSB1C3(top tube)

  • The evidence of RFP in BBa_K2546004

  • Using EcoRI and PstI or XbaI and SpeI to proof that we have complete BBa_K2546004

Improvement

  • Part name:pSB1C3
  • Group: NCHU_Taichung 2018
  • Author: YI-CIAN CHEN
  • Summary: This part is an improvement of BBa_J364007 in pSB1C3. We replace the ori of pSB1C3 with the new ori and replication related proteins to let the part express in our endophyte and broaden its host range.


  • the quantity of GFP/per OD performed

  • [http://2018.igem.org/Team:NCHU_Taichung/Design the Shuttle vectors construction process.]


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