Regulatory

Part:BBa_K2520025

Designed by: Noa Eden   Group: iGEM17_TECHNION-ISRAEL   (2017-10-25)


TRE promoter

In the most commonly used plasmids, the tetracycline response element consists of 7 repeats of the 19bp bacterial TetO sequence separated by spacer sequences. It is the TetO that is recognized and bound by the TetR portion of Tet-On or Tet-Off. The TRE is usually placed upstream of a minimal promoter that has very low basal expression in the absence of bound Tet-Off (or Tet-On).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None