Coding

Part:BBa_K2449013

Designed by: Malte Skovsager Andersen   Group: iGEM17_SDU-Denmark   (2017-09-28)


cenA with a hlyA-tag, with a G-linker

Is two parts consisting of BBa_K2449011 and BBa_K208006 with a G-linker. Giving CenA a Hly-Tag, makes it possible to secret the endoglucanase, using the secretion system BBa_K1166002. It is used in following brick BBa_K2449026 together with cex (exoglucanse) BBa_K2449014.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 325
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 177
    Illegal BamHI site found at 1387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 217
    Illegal AgeI site found at 313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 25


[edit]
Categories
Parameters
None