Composite

Part:BBa_K2285013

Designed by: Zhuoyang Chen   Group: iGEM17_SJTU-BioX-Shanghai   (2017-10-18)


J23119+Target3+amilGFP(with RBS and Term added)

constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilGFP as the reporter gene. The original part of amilGFP chromoprotein is presented on BBa_K592010. What should be noticed is, the original part is a coding part and doesn't have its own RBS and Term. Therefore, to achieve greater convenience fo our Star-chromoprotein reporter system, we add the RBS which is on the upstream of sfGFP(BBa_K515005) as well as a short terminator B1006 to all chromoproteins we used in this project via PCR.

Usage and Biology

The coding region of amilGFP was originated from iGEM11_Uppsala-Sweden.

More infomation and details about usage of this part can refer to J23119+Target3+eforRed BBa_K2285012

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None