Composite

Part:BBa_K214001

Designed by: QGEM_09   Group: iGEM09_Queens   (2009-10-19)


Pconst-RBS-Lpp-OmpA-Linker-TEV-TEV-Linker-ANP

ANP outer membrane expression construct, with twin TEV cut sites designed for release of adhesion. Over expression of sequence can be toxic in bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 639
    Illegal XhoI site found at 540
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None