Part:BBa_K1992007

PctA-Tar expression system (promoter+RBS+coding+terminator)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 331
  Illegal NgoMIV site found at 412
  Illegal NgoMIV site found at 416
  Illegal NgoMIV site found at 435
  Illegal AgeI site found at 535
  Illegal AgeI site found at 746
  Illegal AgeI site found at 1601
  
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

PctA is a chemoreceptor found in the bacterium Pseudomonas Aeruginosa which mediates chemotaxis toward amino acids and away from organic compounds. It is sensitive to all amino acids except for Aspartate (1). A PctA-Tar chimera was formed by the replacement of the Tar LBD with the one of PctA. A procedure which previously was conducted in the literature was recovered (1), in order to prove the concept of the S. Tar platform.

Usage and Biology

Essentially, the expression of the PctA-Tar(BBa_K1992001) chimera receptor is enabled independently by this device, while taking advantage of the bacterium expression resources.

Experiments and results

For methods and results please go to PctA-Tar (BBa_K1992001) or to our [http://2016.igem.org/Team:Technion_Israel/Proof proof of concept] page