Part:BBa_K1861303
CMV Promoter
Human cytomegalovirus (CMV) promoter without enhancer. The sequence includes the binding sites for TALE1 BBa_K1861501 and TALE2 BBa_K1861601.
This part has not been created as a standalone. Instead, it is a component of BBa_K1861030.
Literature Characterization by AFCM-Egypt 2022 team
An essential part originated from the human cytomegalovirus we employ it to regulate the expression of the Cas12g the protein domain of our CRISPR regulatory system CMV promoter is known to have a moderate level of expression lesser than other promoters we take advantage of that to limit the possibility of overexpression of Cas12g to reduce the off targeting effect
The greatest expression levels were seen in the cells transfected with CHEF-1 promoter-carrying vectors, followed by those with HEF-1, CMV mutant, CMV, mouse CMV, CAG, CAG enhancer, and PGK. When the amount of eGFP expression under the CMV promoter was taken into account as 100, the levels of eGFP expression under the PGK, CHEF-1, HEF-1, mouse CMV, CMV mutant, CAG, and CAG enhancer promoters were 218.13, 184.21, 164.33, 49.12, 47.95, and 8.77, respectively.
Experimental Characterization by AFCM-Egypt 2022 team
This figure shows an experimental characterization of this part as it's validated through gel electrophoresis as it is in lane 4. The running part (ordered from IDT) included Human u6 Promoter - -Kinkturn - CMV Promoter - Cas12g.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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