Composite

Part:BBa_K150007

Designed by: Pascal Kraemer   Group: iGEM08_Heidelberg   (2008-10-21)


ColicinE1 operon

This colicinE1 operon contains all three important genes for colicinE1 expression and release. The colE1 gene codes for the lethal colicinE1 protein. The gene for the colE1 immunity protein located on the reverse strand is responsible for the immunity of the producing host bacteria to the colicinE1 and is therefore controlled by a constitutive promoter. Expression of the kil protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 301


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics
//collections/probiotics/biocontainment
Parameters
None