Composite
LacI-T

Part:BBa_K143062

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-01)

LacI repressor protein - Terminator


LacI transcriptional repressor protein (BBa_K143033) coupled to the double terminator (BBa_B0015).


The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with B. subtilis. LacI can be used in conjunction with the lac operon promoter (BBa_K143015), where the LacI will act as a receiver for an IPTG input to result in a Polymerases per second (PoPS) output.


The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.


The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.


Please note. This part has picked up a mutation, which changes an amino acid residue from a Serine to a Leucine but does not cause a frame shift. Given the size of the protein, this should not adversley afect its function to a significant degree.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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