Part:BBa_K143056

Promoter xyl and RBS gsiB for B. subtilis

Inducible promoter xyl(BBa_K143014) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.

Pxyl-gsiB can be used to take an input of xylose and give a Ribosomes per second (RiPS) output generator.

Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator XylR, (BBa_K143036). This means that XylR must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by B. subtilis but should be upregulated to increase efficiency.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

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1. 1 https://parts.igem.org/Assembly:RBS-CDS_issues

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