Generator
Ph-s-gsiB

Part:BBa_K143054

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-08)

Promoter hyper-spank and RBS gsiB for B. subtilis


Inducible promoter hyper-spank(BBa_K143015) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.


Phyperspank-gsiB can be used to take an input of IPTG and give a Ribosomes per second (RiPS) output generator.


IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, (BBa_K143035). This means that LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.


To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio>

  1. 1 https://parts.igem.org/Assembly:RBS-CDS_issues

</biblio>


[edit]
Categories
//chassis/prokaryote/Bsubtilis
Parameters
None