Composite

Part:BBa_K1197004

Designed by: Alişan Kayabölen   Group: iGEM13_METU_Turkey   (2013-09-20)

RBS SpoVG + LacI + DT

This is a LacI producing part which is proper for B.subtilis. Any promoter can be added to the downstream of that part to obtain a LacI coding device.

This part is obtained from BBa_I732820 part by changing the original RBS sequence with RBS SpoVG via specific primers. And LacI was codon optimized for expression in B.subtilis. Original Elowitz RBS does not work well in B.subtilis according to literature researches.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Gel Electrophoresis photo of LacI construct (RBS SpoVG + LacI + DT)

MazF_RBS_LacI_DT.jpg


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
None