Part:BBa_J435371
EF_TZ
Strong triple terminator (UUCG_T7-TΦ , rnnBT1, T7-TΦ), defined as an EF part.
FLANKED 5' fusion site: GCTT, 3' fusion site: CGCT
Source organism: N/A, Target organism: Escherichia coli
ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project
URL: https://freegenes.github.io/genes/BBF10K_003477.html
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
Illegal NotI site found at 149 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
Illegal BamHI site found at 1
Illegal XhoI site found at 158 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7
- 1000COMPATIBLE WITH RFC[1000]
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NYU Abu Dhabi 2024 Characterization
Group:
NYU-Abu-Dhabi 2024 EF_TZ is a terminator sequence that was assembled into a genetic construct using Golden Gate Assembly, allowing for the integration of the terminator in a precise location within the construct, downstream of the eYFP reporter gene. It was tested under two temperature conditions, 28°C and 37°C, to assess its efficiency in ensuring proper transcription termination in bacterial systems. According to the terminator activity graph, the data for EF_TZ at 37°C only extends up to around 3 hours. During this time, EF_TZ exhibited a steady increase in activity, peaking, before the data points ended. There is no data beyond 3 hours at 37°C.
At 28°C, EF_TZ showed a similar trend: the activity increased steadily during the initial hours, peaked around 17 hours, but then started to decline gradually toward the end of the time course. Overall, EF_TZ demonstrated slightly lower activity at 37°C than at 28°C within the recorded time frame. The absence of data past 3 hours at 37°C suggests further analysis may be needed to understand its long-term behavior at higher temperatures. 
Figure 1. Termination activity of EF_TZ at 28°C and 37°C. </center>
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