DNA
pSC101

Part:BBa_I50042

Designed by: Reshma Shetty   Group:   (2007-04-11)

pSC101 origin of replication

BBa_I50042 is a pSC101 origin of replication. BBa_I50042 is the replication origin found in the pSB4*5 series of BioBrick vectors.

Usage and Biology

  • BBa_I50042 yields vectors with an expected copy number of ~5 per cell ([http://openwetware.org/wiki/Molecular_Cloning Molecular Cloning]).

References

Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
[http://www.jbioleng.org/content/2/1/5 URL]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656107 GenBank EU496096]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid/component/origin
Parameters
None